JRC GMO-Amplicons | EURL GMFF

Record ID:	KU291497.1
Database:	nt	Go to original record
Description:	Diheteropogon amplectens var. catangensis voucher PI<ITA>:12585 chloroplast, complete genome.
Taxon:	Diheteropogon amplectens var. catangensis
Original size:	139700 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47755	48283	+	529 bp

Amplicon:

529 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggcaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatccc agaacccata ttataatata
251   ggttctttat tttattttta gaatgaaatt tggaaggatt atgaaataga
301   aaattaataa tttttttaga attattgtga atctattcca atcgaatatt
351   gagtaatcaa atccttcaat tcattgtttt cgagattttt tttttttaaa
401   gtggattaat cggacgagga taaagagaga gtcccattct acatgtcaat
451   actgacaaca atgaaatttc tagtaaaagg aaaatccgtc gactttataa
501   gtcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	529	510	2.38e-08

Match details