JRC GMO-Amplicons | EURL GMFF

Record ID:	KU291494.1
Database:	nt	Go to original record
Description:	Chasmanthium laxum var. sessiliflorum voucher ISC<USA-IA>:Sanchez-Ken s.n. chloroplast, complete genome.
Taxon:	Chasmanthium laxum subsp. sessiliflorum
Original size:	140892 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49054	49670	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttaa aaaaacaagt ggttcttaaa
151   ttagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aggtaattat gttgtgttgg tagtagaact ccctcgaaat
251   tagagaaaga agggctttat acatctaata cacacgtata gatactgaca
301   tagcaaacga ttaatcacag aacccatatc ataatatagg ttctttattt
351   atttattttt tcgaatgaaa ttaggaatga ttatgaaata gaaaattcag
401   aattttttta gaactattgt gaatccattc caatcgaata ttgagtaatc
451   aaatccttca attcattgtt ttcgagatcc tttaaaaagt ggattaatcg
501   gacgaggata aagagagagt cccattctac atgtcaatac tgacaacaat
551   gaaatttcta gtaaaaggaa aatccgtcga ctttataagt tgtgagggtt
601   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details