JRC GMO-Amplicons | EURL GMFF

Record ID:	OR260174.1
Database:	nt	Go to original record
Description:	Hemerocallis citrina isolate 182 chloroplast, partial genome.
Taxon:	Hemerocallis citrina
Original size:	155852 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47976	48562	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aactaaaaat
101   gggcaatcct gagccaaatc tttttttttc aaaaaaggtt tttttttttt
151   gaaactcgaa taaaaaaaga taggtgcaga gactcaatgg aagctgttct
201   aacgaatgga gttgactacg ttgtgttggt agccggaatc cttctatcga
251   aattaaaaaa aggatgacta tatatctaat acatacgtat acatactgac
301   atatcaaacg attaatcatg acccgaatta gtattatata tatgcaaaat
351   tcagagttat tgtggatcta ttccaatcga agttgaagga agaacagaat
401   atttaatgat caaatcattc attccagggt ttgataggcc tttacttttt
451   tttgacaaac tgattaatcg gacgagaata aagagagagt cccattctac
501   atgtcaatac cgacaacaat gaaatttata gtaagaggaa aatccgtcga
551   ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details