JRC GMO-Amplicons | EURL GMFF

Record ID:	OR260146.1
Database:	nt	Go to original record
Description:	Hemerocallis hybrid cultivar cultivar Xueqiukui isolate 92 chloroplast, partial genome.
Taxon:	Hemerocallis hybrid cultivar
Original size:	155837 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48158	48742	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aactaaaaat
101   gggcaatcct gagccaaatc tttttttttt caaaaaaggt ttttttttga
151   aactcgaata aaaaaagata ggtgcagaga ctcaatggaa gctgttctaa
201   cgaatggagt tgactacgtt gtgttggtag ccggaatcct tctatcgaaa
251   ttcaaaaaag gatgactata tatctaatac atacgtatac atactgacat
301   atcaaacgat taatcatgac ccgaattagt attatatata tgcaaaattc
351   agagttattg tggatctatt ccaatcgaag ttgaaggagg aacagaatat
401   ttaatgatca aatcattcat tccagggttt gataggcctt tacttttttt
451   tgacaaactg attaatcgga cgagaataaa gagagagtcc cattctacat
501   gtcaatacgg acaacaatga aatttatagt aagaggaaaa tccgtcgact
551   ttaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details