JRC GMO-Amplicons | EURL GMFF

Record ID:	OR260115.1
Database:	nt	Go to original record
Description:	Hemerocallis hybrid cultivar isolate 22 chloroplast, partial genome.
Taxon:	Hemerocallis hybrid cultivar
Original size:	156184 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47993	48578	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aactaaaaat
101   gggcaatcct gagccaaatc tttttttttc aaaaaaggtt tttttttgaa
151   actcgaataa aaaaagatag gtgcagagac tcaatggaag ctgttctaac
201   gaatggagtt gactacgttg tgttggtagc cggaatcctt ctatcgaaat
251   tcaaaaaagg atgactatat atctaataca tacgtataca tactgacata
301   tcaaacgatt aatcatgacc cgaattagta ttatatatat atgcaaaatt
351   cagagttatt gtggatctat tccaatcgaa gttgaaggaa gaacagaata
401   tttaatgatc aaatcattca ttccagggtt tgataggcct ttactttttt
451   ttgacaaact gattaatcgg acgagaataa agagagagtc ccattctaca
501   tgtcaatacc gacaacaatg aaatttatag taagaggaaa atccgtcgac
551   tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details