JRC GMO-Amplicons | EURL GMFF

Record ID:	OR344069.1
Database:	nt	Go to original record
Description:	Gynostemma pentaphyllum voucher sxk1 chloroplast, complete genome.
Taxon:	Gynostemma pentaphyllum
Original size:	157721 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49655	50263	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaatca aataagggct
151   tcgaaagcga gaaaaaaagg gtaggtgcag agactcaatg gaagctgttc
201   taacaaatga gttgactagg ttgcgttagt aaaggaatga atccttccat
251   cgaaactcca gaaaagatga aagataaacg tatatacgta ctgaaatact
301   atttgattaa tgacagcccg aatctctatt ttttatagtt atctaaaaaa
351   tgatatatat atatataaaa cgaattgtta ttaatcgatt ccaatatctc
401   caggttgaaa aaagaatcga atattcattg atcaaatcat ttactccatc
451   atagtctgat agatcttttg aagaactgat taatcagatg agaatagaat
501   aaagatagag tcccattcta catgtcaata ccgacaaaaa tgaaatttat
551   agtaagagga aaatccgtcg acttaaaaaa atcgtgaggg ttcaagtccc
601   tctatcccc

View other 6 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details