JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ434193.1
Database:	nt	Go to original record
Description:	Galium trifidum isolate glu125 chloroplast, complete genome.
Taxon:	Galium trifidum
Original size:	154795 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48313	48909	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acctgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggtttagaa
151   agtgaaaaag gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttcac tgtgttagtc gagaattctt tccatcaaaa attcagaaaa
251   tacaagtatt aagtgaagga taaacgtata tactatccat aacgtagtga
301   atagtatatt aaatgagtaa tgacagcccc aacgaatcta tactttttca
351   tagaaaaaat atagaattgt aattgttgtt aaaatattcc acgtttaaga
401   aataagaaag aatcgaatat tcatttatca aatgattcac tccatagtct
451   gatagatctt tttacgaact gattaatcgg acgagaataa agatagagtc
501   ccattctaca tgtcaatgcc ggcagcaatg aaatttatag ttaagaggaa
551   aatccgtcga cttttaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details