Match details
Record ID: |
CP126427.1 |
Database: |
nt |
Go to original record |
Description: |
Glycine max cultivar Williams 82 chromosome 2. |
Taxon: |
Glycine max |
Original size: |
54744224 bp |
View other 10 matches for this record
Method: |
QT-TAX-GM-009 |
Go to GMOMETHODS record |
Description: | Quantitative PCR method for detection of soybean lectin gene (Mazzara et al., 2007). |
Target: | soybean lectin |
View other 11 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
2 |
0 |
1139576 |
1139680 |
+ |
105 bp |
|
1 cttctttctc gcaccaattg acactaagcc acaaacacat gcaggttatc
51 ttggtctttt caacgaaaac gagtctggtg atcaagtcgt cgctgttgag
101 tttga
View other 11 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
20 bp |
0 |
0 |
4 |
23 |
6.31e-09 |
primerB |
100.0 |
20 bp |
0 |
0 |
105 |
86 |
5.26e-09 |
probe |
100.0 |
26 bp |
0 |
0 |
29 |
54 |
3.40e-12 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.