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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: CP126427.1
Database: nt Go to original record
Description: Glycine max cultivar Williams 82 chromosome 2.
Taxon: Glycine max
Original size: 54744224 bp

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Method: QT-TAX-GM-002 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of soybean lectin gene
Target:soybean lectin

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1139506 1139579 + 74 bp
Amplicon: 74 bp 
1     ccagcttcgc cgcttccttc aacttcacct tctatgcccc tgacacaaaa
51    aggcttgcag atgggcttgc cttc

View other 25 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 3.63e-09
primerB 100.0 23 bp 0 0 74 52 9.36e-11
probe 100.0 24 bp 0 0 23 46 2.75e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.