JRC GMO-Amplicons | EURL GMFF

Record ID:	ON229584.1
Database:	nt	Go to original record
Description:	Persicaria maackiana voucher SD307 chloroplast, complete genome.
Taxon:	Persicaria maackiana
Original size:	160682 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48627	49222	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaagaa
101   atgggcaatc ctgagccaac tcctgctttc caaaaggaaa gaaaaagagg
151   ataggtgcag agactcaatg gaagctgttc taacaaatgg aattgactgt
201   cttgcgttgt aatatgttat aagaattttt cgatctaaac tccaaaagtg
251   atgaagaaga aacctatagg tatacataaa tagaaagact atactatgat
301   ataataaata aagaatgcaa aaatattgat gacgaaccga attttttttt
351   atgaatagga aaaaaatgga agaatttttg tgaatcgatt ccaagttgaa
401   gaaagaatcg aatattcgtt tattaaatta aagcatttac tccacagttt
451   ggtagatctt ttgaagaacc aacccatcgg atgagaatga agatagagtc
501   ccattctacg tgtcaatccc gacaccaatg aaatttatag taagaggaaa
551   atccgtcgac tttataaatc gtgagggttc aagtccctct atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details