JRC GMO-Amplicons | EURL GMFF

Record ID:	ON229571.1
Database:	nt	Go to original record
Description:	Persicaria filiformis voucher SD250 chloroplast, complete genome.
Taxon:	Persicaria filiformis
Original size:	159721 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47992	48588	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaaa
101   atgggcaatc ctgagccaac tcctgctttc caaaaggaaa gaaaaaaggg
151   ataggtgcag agactcaatg gaagctgttc taacaaatgg aattgactgt
201   cttgcgttgt aatatgttat aagaattctt cgatcgaaac tccaaaaatg
251   atgaagaaga aacctatagg catacgtact taaatagaaa gactatacta
301   tgatataata aaaaatgaaa aaatattgat gacgacccga acttttattt
351   tatgaatatg aaaaaaatgg aataattttt gtgaatcgat tccaagttga
401   agaaagaatc gaatattcgt ttattaaatt aaagcattta ctccacagtt
451   tggtagatct tttgaagaac cgatccatcg gatgagaatg aagatagagt
501   cccattctac gtgtcaatcc cgacaacaat gaaatttata gtaagaggaa
551   aatccgtcga ctttataaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details