JRC GMO-Amplicons | EURL GMFF

Record ID:	ON229566.1
Database:	nt	Go to original record
Description:	Persicaria amphibia voucher SD638 chloroplast, complete genome.
Taxon:	Persicaria amphibia
Original size:	159464 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47757	48360	+	604 bp

Amplicon:

604 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaaa
101   tgggcaatcc tgagccaact cctgttttca aaaaggaaag aaaaaaggga
151   taggtgcaga gactcaatgg aagctgttct aacaaatgga attgactgtc
201   ttgcgttgta atatgttata agaattcttc gatctaaacg ccaaaaagga
251   tgaagaagaa acctataggc ataggtactt aaatagaaag actatactat
301   gatataataa aataagaaaa aatgcaaaaa tattgatgac gaaccgaatt
351   tttattttat gaatatgaaa aaaatggaag aatttttgtg aatcgattcc
401   aagttgaaga aagaatcgaa tattcgttta ttaaattaaa gcatttcctc
451   cacagtttgg tatatctttt gaagaaccga tccatcggat gagaatgaag
501   atagagtccc attctacgtg tcaatcccga caacaatgaa atttatagta
551   agaggaaaat ccgtcgactt tataaatcgt gagggttcaa gtccctctat
601   cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	604	585	2.72e-08

Match details