JRC GMO-Amplicons | EURL GMFF

Record ID:	LC775283.1
Database:	nt	Go to original record
Description:	Callicarpa dichotoma HIRO:HIRO-MY-156564 chloroplast DNA, complete genome.
Taxon:	Callicarpa dichotoma
Original size:	154110 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47956	48520	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgactgcgtt ggtagaggaa tctttccatg gaaacttcag
251   aaaggatgaa ggataaacgt atctattgaa tactatatca aatgattaat
301   gatggcccga atctgtatct gtatttttga atatgaaaaa tggaagaatt
351   ggtgtgaatt gattccacat tgaagaaaaa atcgaatatt cattcatcaa
401   atcattcact ccataggccg atagatcttt taaagaactg attaatcgga
451   cgagaataaa gatagagtcc cattctacat gtcaataccg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details