JRC GMO-Amplicons | EURL GMFF

Record ID:	OR260539.1
Database:	nt	Go to original record
Description:	Ceropegia sp. PL-2023a chloroplast, complete genome.
Taxon:	Ceropegia sunhangiana
Original size:	161376 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48990	49546	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatt ctgagccaaa tcctattttc aggttcggaa aacgaacaca
151   aggataggtg cagagactcg acggaagctg ttctaacaaa tggagttggc
201   cgcgttggta gagaaatctt tccatcgaaa attcagaaag gatgaaagat
251   atacatacgt attgaatact atagaaaatg attaatgccg atccaaatga
301   gtccgttatt ttctataaaa aacggaagaa ttggtgtgat tcgattccac
351   attgaagaaa gaatcgaata ttcattgatc aaaagattca ctccatagtc
401   tgtagatcct cttttcaaga accggattaa tcggacgaga ataaagaata
451   aagatagagt cccgttctac atgtcaatgc tggcaacaat gcaattttga
501   gtaagaggaa aatccgtcga cttaaaaaat cgtgagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details