JRC GMO-Amplicons | EURL GMFF

Record ID:	OP136026.1
Database:	nt	Go to original record
Description:	Dianthus barbatus chloroplast, complete genome.
Taxon:	Dianthus barbatus
Original size:	149642 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46552	47171	+	620 bp

Amplicon:

620 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aataaaaaaa
101   agggtaatcc tgagccaact ccttttgttt ccttttttca aaagaaaaaa
151   aggataggtg cagagactca aaggaagctg ttctaacaaa ttgggttgac
201   tgtgctgtgt tgttataaga cttcttccgt ctaaattccc ataaaaaagg
251   gggggtaaaa tcgacggagt gaaatgatta atgacaaccc gaatctgttc
301   tgtatttttt tttatatatt atatatatat aaatccatac tttattctat
351   atttagagta gagatctaga agattaaatg aaaaaatgca agaattcttg
401   tgaatccatt caaagttgaa agcagaatca atcaagattt attcattaaa
451   atattcacac taactccata gtctgataga tattgtgaag aacttattaa
501   tcggatgaga ataaagatag agtcccgtac tacatgtcaa tactgacaac
551   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcatgagg
601   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	620	601	2.79e-08

Match details