JRC GMO-Amplicons | EURL GMFF

Record ID:	KU974011.1
Database:	nt	Go to original record
Description:	Cruciata glabra tRNA-Phe (trnF), trnF-trnL intergenic spacer and tRNA-Leu (trnL), region; chloroplast.
Taxon:	Cruciata glabra
Original size:	549 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	548	+	547 bp

Amplicon:

547 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acctgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa atcctatttt tcgaaaacaa aggtttagaa
151   aatgaaaaag gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttcgc tgtgttagtc gataattctt tccatcaaaa actcagaaaa
251   tacaagtact aagtattaag tgaaggataa atgtatataa cgagtatatt
301   ttaacgagta tattttatat taaattatta aatgagtaat gacagcccca
351   atgaatctat actttttcat agaaaaaatt aacgattcac tccatagtct
401   gatagatctt tttacgaact gattaatcgg acgagaataa agatagagtc
451   ccgttctaca tgtcaatgcc ggcaacaatg aaatttatag ttaagaggaa
501   aatccgtcga cttttaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	547	528	2.46e-08

Match details