JRC GMO-Amplicons | EURL GMFF

Record ID:	KR704025.1
Database:	nt	Go to original record
Description:	Streptocarpus tanala voucher Moeller M. & Rafanantsoa G. MM9710 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Streptocarpus tanala
Original size:	917 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	545	+	545 bp

Amplicon:

545 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atccttgttt tctcaaaaca agggttcaga
151   gaacgaaaaa gaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgttggt agaggaattg aaacttcaga aaggatgaat
251   agtatctatt gaatactatt gatgattaat aatggcccga atctgtatct
301   gggtttttgt atatgaaaaa tggaagaatt ggtgtgaatt gatttcacat
351   tgataaaaga atcgaatatt cattgatcaa ataattcacc ccatagtccg
401   atagatctct taaagaattg attagtcgga cgagaataaa gatagagtcc
451   cgttctacat gtcaataccg acaacaatga aacttatagt aagaggaaaa
501   tccgtcgact ttaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	545	526	2.45e-08

Match details