JRC GMO-Amplicons | EURL GMFF

Record ID:	KR704020.1
Database:	nt	Go to original record
Description:	Streptocarpus schliebenii voucher Burtt B.L. s.n. tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Streptocarpus schliebenii
Original size:	948 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	573	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atccttgttt tctcaaaaca agggttcaga
151   gagcgaaaaa gaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgtcggt agaggaattg aaacttcaga aaggatgaaa
251   cgtatctatt gaatacatta ataamgtatc tattgaatac attattgaat
301   acattaataa tggcccgaat ctgtatctgg gtttttgtat atraaaaatg
351   gaagaattgg tgtgaattga tttcacattg ataaaagaat cgaatattca
401   ttgatcaaat aattcacccc atagtccgat agatctctta aagaattgat
451   tattcggacg agaataaaga tagagtcccg ttctacatgt caataccggc
501   aacaatgaaa cttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg
551   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details