JRC GMO-Amplicons | EURL GMFF

Record ID:	KR704019.1
Database:	nt	Go to original record
Description:	Streptocarpus saxorum voucher Mather S. 1330 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Streptocarpus saxorum
Original size:	903 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	536	+	536 bp

Amplicon:

536 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa ctccttgttt tctcaaaaca agggttcaga
151   gagcgaaaaa gaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgtgttggt agaggaattg aaacttcaga aaggatgaaa
251   cgtatctatt gatcattaat aatggcctac atctgtatct gggtttttgt
301   atatgaaaaa atggaagaat tggtgtgaat tgatttcaca ttgataaaag
351   aatcgaatat tcattgatca aatcattgac cccatagtcc gatagatctc
401   ttaaagaatt gattattcgg acgagaataa agatagagtc ccgttctaca
451   tgtcgatacc gacaacaatg aaacttatag taagaggaaa atccgtcgac
501   tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.41e-08
primerB	100.0	20 bp	0	0	536	517	2.41e-08

Match details