JRC GMO-Amplicons | EURL GMFF

Record ID:	KR703966.1
Database:	nt	Go to original record
Description:	Streptocarpus exsertus voucher Bellstedt D.U. & Bytebier B. 564 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Streptocarpus exsertus
Original size:	887 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	544	+	544 bp

Amplicon:

544 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atccttgttt tctcaaaaca agggttcaga
151   gagcaaaaaa gaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttgc ctgcattggt agaggaattg aaacttcaga aaggatcaaa
251   cgtatctatt gaatacatta ctaatgacta atggcccgaa tctgtatctg
301   ggtttttgta tatgaaaaat ggaagaattg gtgtgaattg atttcacatt
351   gataaaagaa tcgaatattc attgatcaaa tcattcaccc catagtccga
401   tagatctctt aaagaattga ttattcggac gagaataaag atagagtccc
451   gttctacatg tcaataccgg caacaatgaa acttatagta agaggaaaat
501   ccgtcgactt taaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	544	525	2.45e-08

Match details