JRC GMO-Amplicons | EURL GMFF

Record ID:	KR703953.1
Database:	nt	Go to original record
Description:	Streptocarpus capuronii voucher Moeller M. & Andriantiana J. MM9885A tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Streptocarpus capuronii
Original size:	912 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	537	+	537 bp

Amplicon:

537 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atccttgttt tctcaaaaca agggttcaga
151   gaccgaaaaa gaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgttggt agaggaattg aaacttcaga aaggatgaaa
251   cgtatctatt gaatacatta ataatggccc gaatctgtag ctgggttttt
301   gtatattaaa aatggaagaa ttggtgtgaa ttgatttcac attgataaaa
351   gaatcgaata ttcattgatc aaattattca ccccatagtc cgatagatct
401   cttaaagaat tgattattcg gacgagaata aagatagagt cccgttctac
451   atgtcaatac cggcaacaat gaaacttata gtaagaggaa aatccgtcga
501   ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.42e-08
primerB	100.0	20 bp	0	0	537	518	2.42e-08

Match details