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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: OY292386.1
Database: nt Go to original record
Description: Verbena officinalis genome assembly, chromosome: 2
Taxon: Verbena officinalis
Original size: 46094953 bp

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Method: QL-ELE-00-010 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Figwort Mosaic Virus 35S promoter (Pan et al., 2007).
Target:P-FMV

View other 425 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 2 37979369 37979526 - 158 bp
Amplicon: 158 bp 
1     ccaagaccaa catggtcatg gtcttcggtg agatcaccac caaggctaat
51    gttgactacg agaaaattgt acgtgagaca tgccgcaaca ttggattcat
101   ttctgatgat gttggcttgg atgccgacaa ctgcaaggtt cttgtcaaca
151   tcgagcag
Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerB 100.0 18 bp 0 0 158 141 9.02e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.