JRC GMO-Amplicons | EURL GMFF

Record ID:	KT716391.1
Database:	nt	Go to original record
Description:	Enterococcus faecalis strain 3EF plasmid pJ3EF transposon Tn6010-like insertion sequence IS26, complete sequence, hypothetical protein, OqxA membrane-fusion protein (oqxA), OqxB integral membrane protein (oqxB), and transcriptional regulator genes, complete cds, and insertion sequence IS26, complete sequence; and transposon Tn5 bleomycin resistance protein (ble) gene, partial cds, and aminoglycoside-3'-O-phosphotransferase-IIa (aph(3')-IIa) gene, complete cds.
Taxon:	Enterococcus faecalis
Original size:	8370 bp

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Method:	QL-ELE-00-002	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:	nptII

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	7487	7701	-	215 bp

Amplicon:

215 bp

1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	21 bp	0	0	1	21	3.06e-09
primerB	100.0	21 bp	0	0	215	195	3.06e-09

Match details