JRC GMO-Amplicons | EURL GMFF

Record ID:	KU726550.2
Database:	nt	Go to original record
Description:	Bienertia sinuspersici chloroplast, complete genome.
Taxon:	Bienertia sinuspersici
Original size:	153472 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47417	48017	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa ctcttttttt tttgcttttg aaaaaaaaag
151   gataggtgca gagactcaaa ggaagctgtt ctaacaaatg ggtttgactg
201   tgttgttcta agaattcttc cgtcaaaact ccaatccaaa aagggtgaag
251   aatatactat gtcaaatcat tcatgacacc ctgaatctat tttgtatttt
301   tttctaattt tttgatattt cttatttctc tatttaatag agaaaatgaa
351   attcttgcta aataaaaatt gagaatatga aatgaaaata tatatatcat
401   atgaattgga aaaggggaag aatttttgtg aatcaatttc aagttgaaaa
451   aagaatcaaa tattcattca tcaaatcatt cactcccgag aataaagata
501   gagtcccgtt ttacatgtca atactgacaa gaatgaaatt tatagtaaga
551   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
601   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details