JRC GMO-Amplicons | EURL GMFF

Record ID:	KP901228.1
Database:	nt	Go to original record
Description:	Prolobus nitidulus voucher R.P.Oliveira 1366 (HUEFS) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prolobus nitidulus
Original size:	863 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	525	+	525 bp

Amplicon:

525 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   agtgggcaat cctgagccaa atcacgtttt ccgaaaacaa acaaaggttc
151   agaaagcgaa aataaaaaag gataggtgca gagactcgat ggaagctgtt
201   ctaacgaatg gagttgattg tcttacattg gtagaggaat ccttctatcg
251   aaacttcaga aaagatgaag gataaacctg tatacataga agaattggtg
301   tgaatcgatt ccatattgaa gaaagaatca aatcttcatt gatcaaagca
351   ttcacgcata atctgattca cgcaaaatct gtagatcttt tgaagaactg
401   attaatcgga cgagaataaa gatagagtcc cgttctacat gtcaataccg
451   gcaacaatga aatttatagt aagaggaaaa tccgtcgatt tcaaaaatca
501   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.36e-08
primerB	100.0	20 bp	0	0	525	506	2.36e-08

Match details