JRC GMO-Amplicons | EURL GMFF

Record ID:	KU724137.1
Database:	nt	Go to original record
Description:	Haplostachys linearifolia strain JFR14025/JFR7025 chloroplast, partial genome.
Taxon:	Haplostachys linearifolia
Original size:	150129 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45914	46476	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaan
101   nnnnnnnnnn nnncgatcct gatccaaatc ctgttttttc aaaacaatgg
151   ttcaaaaaac caaataaagg ataggtgcag agactcaacg gaagctgttc
201   taacaaatgg agttgactgc gttggtagag gaatcctttc tacggaaact
251   tcagaaagga tgaaggataa acgtatctat cgaatactat atcaaatgat
301   taatgatggt ccgaatccgt atttttgaat atgaaaaata gaagaattgg
351   tgtgaattga ttctatattg aagaaaaaat cgaatattca ttcatcaaat
401   cattcactcc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
451   nnnnnnnnnn nnnagtccca ttctacatgt caataccggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details