JRC GMO-Amplicons | EURL GMFF

Record ID:	KU724131.1
Database:	nt	Go to original record
Description:	Phyllostegia waimeae strain HI0327 chloroplast, partial genome.
Taxon:	Phyllostegia waimeae
Original size:	150137 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45903	46470	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaat
101   taatcaaaat gggcgatcct gatccaaatc ctgttttgtt ttttcaaaac
151   aatggttcaa aaaaccaaat aaaggatagg tgcagagact caacggaagc
201   tgttctaaca aatggagttg actgcgttgg tagaggaatc ctttctacgg
251   aaacttcaga aaggatgaag gataaacgta tctatcgaat actatatcaa
301   atgattaatg atggtccgaa tccgtatttt tgaatatgaa aaatagaaga
351   attggtgtga attgattcta tattgaagaa aaaatcnaat attcattcat
401   caaatcattc mctccatagt ccgatagatc ttttaaagac ctrattaatc
451   ggacgmgaat aaagatagag tcccattcta catgtcaata ccggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details