JRC GMO-Amplicons | EURL GMFF

Record ID:	OY282506.1
Database:	nt	Go to original record
Description:	Ajuga chamaepitys genome assembly, chromosome: 12
Taxon:	Ajuga chamaepitys
Original size:	57120494 bp

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Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	37132107	37132660	-	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg aacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataagttt caaattcaga gaaacctagg aactgataaa
101   aatgggcaat cttgagccaa atcatgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa gggataggtg cagagactca atggaagttg ttctaacaaa
201   tggagttcaa tgcgctggta gaggaatctt ttcatagaaa attcagaaag
251   gatgaaggat aaatggatct attgaatact atatcgaatg attcacgatg
301   gcccgaatct gtatttttaa tatgaaaaat ggaagaattg gtgtgaattg
351   attccatatt gaagaaaaaa tcgaatattc attcatcaaa tcattcactc
401   catagtccga tagttctttt caagaactga ttaatcgaac gagaataaag
451   atagagtccc attctacatg tcaataccgg caacaatgaa atttatggta
501   agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
551   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details