JRC GMO-Amplicons | EURL GMFF

Record ID:	OY282505.1
Database:	nt	Go to original record
Description:	Ajuga chamaepitys genome assembly, chromosome: 11
Taxon:	Ajuga chamaepitys
Original size:	57420611 bp

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Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	5779838	5780392	-	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataacttt caaattcaga gaaacccagg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttcac tgcgctggta gaggaatctt ttcatagaaa cttcagaaag
251   gatgaaggat aaatggatct attgaatact atatcgaatg attcacgatg
301   gcccgaatct gtatttttta atatgaaaaa tggaagaatt ggtgtgaatt
351   gattccacat tgaagaaaaa atcgaatatt cattcatcaa atcattcact
401   ccatagtccg atagttcttt tcaagaactg attaatcgga cgagaataaa
451   gatagagtcc cgttctacat gtcaataccg gcaacaatga aatttatagt
501   aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca agtccctcta
551   tcccc

View other 10 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details