JRC GMO-Amplicons | EURL GMFF

Record ID:	OX940973.1
Database:	nt	Go to original record
Description:	Euphrasia confusa genome assembly, organelle: plastid:chloroplast
Taxon:	Euphrasia confusa
Original size:	144975 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48299	48833	+	535 bp

Amplicon:

535 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaat
101   aatgggcaat cctgagccaa atcctgtttt ttccaaacaa agattcagaa
151   aacgaaaaaa aggataggtg cagagactct atggaagttg ttctaacaaa
201   tggagttgat tacgctggta gcgggatgaa actattgact actataatca
251   atagattgat taatgaaatg atggcccaaa tctgtatctg tatttttttt
301   tttgaatacg caaaatggaa gaattggtgt gaattgattc catattgaaa
351   aaagaagatt cattgatcaa ataattcact ccatagtccg atcgatcgtt
401   taaagaactg attaatcggg cgagaataaa gatagagtcc cattctacat
451   gtcaataccg acaacaatga aatttatagt aagaggaaaa tccgtcgact
501   tttaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.41e-08
primerB	100.0	20 bp	0	0	535	516	2.41e-08

Match details