JRC GMO-Amplicons | EURL GMFF

Record ID:	OX940726.1
Database:	nt	Go to original record
Description:	Scutellaria minor genome assembly, chromosome: 2
Taxon:	Scutellaria minor
Original size:	36386000 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	10646964	10647520	-	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaatta gattgaactt gatgtgggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtgat cctgatccaa atcctgtttt ctgaaaacaa aggctcaaaa
151   aacgaaaaat gggataggtg cagagactcg atggaagttg ttctaacaaa
201   tggagttagc tgagttgtta gaggaatcct ttccgcggaa acttcagaaa
251   ggatgaagga taaatgtatc tattgaatac tatatcaaat gattaatgat
301   ggcccgaatc tgtatttttg aatatgaaaa acggaagaat cggtgtgaat
351   tgattccaca ttgaaaaaaa aaatggaata ttcattcatc aaatcattca
401   ctccacggtc caataggtct tttaaagaac taattaatcg gacgagaata
451   aagatagagt cccattctac atgtcaatac cggcaacaat gaaatttata
501   gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details