Match details
| Record ID: |
ON773610.1 |
| Database: |
nt |
Go to original record |
| Description: |
Binary vector pFGC-proCas9 GBSS targeting CRISPR/Cas9 vector, complete sequence. |
| Taxon: |
Binary vector pFGC-proCas9 |
| Original size: |
13733 bp |
View other 12 matches for this record
| Method: |
QL-ELE-00-012 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008). |
| Target: | CaMV P-35S |
View other 15625 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
4635 |
4716 |
+ |
82 bp |
|
1 gcctctgccg acagtggtcc caaagatgga cccccaccca caaggagcat
51 cgtggaaaaa gaagacgttc caaccacgtc tt
View other 21 matches with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
18 bp |
0 |
0 |
1 |
18 |
4.54e-08 |
| primerB |
100.0 |
22 bp |
0 |
0 |
82 |
61 |
3.55e-10 |
| probe |
100.0 |
21 bp |
0 |
0 |
21 |
41 |
1.28e-09 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
