JRC GMO-Amplicons | EURL GMFF

Record ID:	OX596242.1
Database:	nt	Go to original record
Description:	Salicornia ramosissima genome assembly, organelle: plastid:chloroplast
Taxon:	Salicornia ramosissima
Original size:	153299 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47604	48199	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaatta gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaaa
101   aaagggcaat cctgagccaa ctcctttttt caaaataaaa aaaggatagg
151   tgcagagact caaaggaagt tgttctaaca aatgggtttg ggttgactgt
201   gttgttctaa gaatccttcc gtcaaaactc caatctaaaa agggtgaaga
251   atatactatg tcaaatgatt aatgacaccc cgaatctata tctattttgt
301   attttttttc taatttttta gtatttatta tttctatatt taatacagaa
351   gatgaaattc tcgctaaata aaaattgcga atatgaaatg aaaatattat
401   aagaaaaaaa gggaagaatt tttgtaaatt gattttaaat tgaaaaagaa
451   tcaaatattc attcatcaaa tcattcactc ccgagaataa agatagagtc
501   ccattctaca tgtcaatact gacaagaatg aaatttatag taagaggaaa
551   atccgtcgac tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details