JRC GMO-Amplicons | EURL GMFF

Record ID:	OP580997.1
Database:	nt	Go to original record
Description:	Trema cannabinum var. dielsianum chloroplast, partial genome.
Taxon:	Trema cannabina var. dielsianum
Original size:	157795 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49820	50414	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccccgg aattaaaaaa
101   atggggtaat cctgagccaa atccggtttt ctgaaaacaa acaaggattc
151   cgaaagcgat aataaaaaag aataggatag gtgcagagac tcaatggaag
201   ctgttctaac aaatggagtt ggctgcgttg cgttaataaa ggaatccctt
251   ccatcgaaac tccagaaacg atgaagaata aacgtatata cgtacaaaaa
301   tcctatctcc aaagaattaa tgaaaacccg aatccgtatc cgtatttctt
351   ttaattttca tgaaaaagga aagaattgtt gtgaatcaat tttaagttga
401   aaaacgaatc gaatattcat tgatcaaatc atttactcca tcaaaatctg
451   atagatcttt tgaagacttg attaatcgga tgagaataaa gatagagtcc
501   cattctacat gtcaatattg ataagaatga aatttatagt aagaggaaaa
551   tccgtcgact ttaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details