JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ873407.1
Database:	nt	Go to original record
Description:	Panicum virgatum chloroplast, complete genome.
Taxon:	Panicum virgatum
Original size:	139577 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48250	48814	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtacggaa
51    acctgctaag tgataacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg taatggaact ccctcgaaat
251   tatagaaaga agggctttat acatctaata cacacgtata gatactcaga
301   tagcaaacga ttaatcatag aacccatatc ataatatagg ttctttattt
351   tattttttag aattattgtg aatccattcc aatcgaatat tgagtaatca
401   aatccttcaa ttcattgttt tgagatcttc aaaaaagtgg attaatcgaa
451   cgaggataaa gagagagtcc cattctacat gtcaatactg acaacaatga
501   aatttatagt aaaaggaaaa tccgtcgact ttataagtcg tgagggttca
551   agtccctcta tcccc

View other 16 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details