JRC GMO-Amplicons | EURL GMFF

Record ID:	KC539179.1
Database:	nt	Go to original record
Description:	Solanum pumilum isolate LB3339 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Solanum pumilum
Original size:	1755 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	674	1246	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa acaaaggttc
151   agaaaaaaag gataggtgca gagactcaat ggaagctatt ctaacaaatg
201   gagttaaatg cgttggtaga ggagactctt tacatcgaaa cttcagaaag
251   aaaaagaatg aagtgaagga taaacgtata tacatacgta ttgaatacta
301   tatcaaatga ttaatgacga cccgaatccg taatttttct ataaaaaata
351   gaagaattgg tgtgaatcca ttctacattg aagaaagaat cgaatattca
401   ttgatcaaat cattcactcc atagtctgat agatcttttg aagaactgat
451   taatcggacg agaataaaga tagagtcccg ttctacatgt caatactggc
501   aacaatgaaa tttatagtaa aaggaaaatc cgtcgacttt aaaaatcgtg
551   agggttcaag tccctctatc ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details