JRC GMO-Amplicons | EURL GMFF

Record ID:	KC539167.1
Database:	nt	Go to original record
Description:	Solanum comptum isolate LB3342 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Solanum comptum
Original size:	1769 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	670	1246	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctatttt ctcaaaacaa acaaaggttc
151   agaaaaaaag gataggtgca gagactcaat ggaagctatt ctaacaaatg
201   gagttaaatg cgttggtaga ggactcttta catcgaaact tcagaaagaa
251   aaagaatgaa gtgaaggata aacgtatata catacgtatt gaatactata
301   tcaaatgatt aatgacgacc cgaatccgaa tccgtaattt ttctataaaa
351   aatagaagaa ttggtgtgaa tccattctac attgaagaaa gaatcgaata
401   ttcattgatc aaatcattca ctccatagtc tgatagatct tttgaggaac
451   tgattaatcg gacgagaata aagatagagt cccgttctac atgtcaatac
501   cggcaacaat gaaatttata gtaaaaggaa aatccgtcga ctttaaaaat
551   cgtgagggtt caagtccctc tatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details