JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ842967.1
Database:	nt	Go to original record
Description:	Paraphlomis koreana voucher KIMJH22027 plastid, complete genome.
Taxon:	Paraphlomis koreana
Original size:	152128 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46779	47341	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc cactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttttc aaaacaaagg
151   ttcaaaaaac gaaataaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgc gttggtagag gaatccttta tacggaaact
251   tcagaaagga tgaaggataa acgtatctat cgaatactat atcaaatgat
301   taatcatggc ccgaatccgt attttttaat atgaaaaata gaagaattgg
351   tatgaattga ttctatattg aagaaaaaat cgaatattca ttcatcaaac
401   cattcactcc atagtccgat agatctttta aagaactgat taatcggacg
451   agaataaaga tagagtccca ttctacatgt caatactggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details