JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ848588.1
Database:	nt	Go to original record
Description:	Ripogonum scandens isolate Rs2 chloroplast, complete genome.
Taxon:	Ripogonum scandens
Original size:	160278 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48974	49573	+	600 bp

Amplicon:

600 bp

1     cgaaatcggt agacgctacg gacttgatta gattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaacaagg gtttaaacta
151   gaatcaaaaa aaggataggt gcagagactc aatggaagct gttctaacga
201   atggagttga ctgcgttacg ttgcgttggt agctggaatc cttctatcaa
251   aattgcagag aggatggccc tatatacgta tatatacata ctgacatatc
301   aaacgattga ttaatcatgg cccgaatcca tatttgatat catatatata
351   tgtgatatat atatataaat taagagagtt attgtgaatc tattccaatc
401   gaagttgaag gaagaatcga atattcagtg ataaaattat tcattccaga
451   gtctggtaga tcttttgaaa aactgattaa tcggacgaga ataaagagag
501   agtcccattc tacatgtcaa taccgacaac aatgaaattt atagtaaaag
551   gaaaatccgt cgactttaga agtcgtgagg gttcaagtcc ctctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	600	581	2.70e-08

Match details