JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ848581.1
Database:	nt	Go to original record
Description:	Lapageria rosea chloroplast, complete genome.
Taxon:	Lapageria rosea
Original size:	160054 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48685	49294	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttgatta gattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaacaagg ctttaattta
151   taaaactaga atcaaaaaag ggataggtgc agagactcaa tggaagctgt
201   tctaacgaat ggagttgact acgttacgtt gcgttggtag ctggaatcct
251   tctatcaaaa ttgcggagag gatggcccta tatacgtata tatacatact
301   gacatatcaa acgattgatt aatcatggcc cgaatccata tttgatatca
351   tatatatata tatatgatat atatatataa attaagagtt attgtgaatc
401   tattccaatc gaagttgaag gaagaatcga atattcagtg ataaaattat
451   tcattccaga gtctggtaga tcttttgaaa aactgattaa tcggacgaga
501   ataaagagag agtcccattc tacatgtcaa tactgacaac aatgaaattt
551   atagtaaaag gaaaatccgt cgactttaga agtcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details