JRC GMO-Amplicons | EURL GMFF

Record ID:	MN848249.1
Database:	nt	Go to original record
Description:	Justicia adhatoda chloroplast, complete genome.
Taxon:	Justicia adhatoda
Original size:	149503 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46435	46997	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aaagggcaat cctgagccaa atcctgtttt cgcaagacaa aggttcagaa
151   aacgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttggct gcattggtag aggaatcgtt ccatcaaaac ttccgaaagg
251   ataaacgtat ctattgaata ttatatcaac agattagatg gcccacatct
301   gtattttgta tttgtatttt tttatatgaa aaatggaaga attgatgtga
351   attgattcca cattgaagaa agagtcgaat attcattcat caaatcattc
401   actccatagt ctaatagttc ttttatttta aagaattaat taagcggacg
451   agaataaaga tagagtccca ttccacatgt caataccggc aacaatgaaa
501   tttagagtaa gaggaaaatc cgtcgacttt caaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details