JRC GMO-Amplicons | EURL GMFF

Record ID:	MN848245.1
Database:	nt	Go to original record
Description:	Justicia procumbens chloroplast, complete genome.
Taxon:	Justicia procumbens
Original size:	150471 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46181	46742	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aaagggcaat cctgagccaa atcctctttt cgcaagacaa aggttcagaa
151   aacaaaaaag ggataggtgc agagactcaa tggaagctgt tctaacttgg
201   ctgcattggt agaggaatcg ttccgtcaaa acttccgaaa ggataaatct
251   atctattgaa tattatatca acagattaga tggcccacat ctgcattttg
301   tatttttttg tatgaaaaat ggaagaattg atgtgaattg attccacatt
351   gaagaaagag tcgaatattc atttattcat tcaccaaaca tcaaatcatt
401   cactccatag tctaatagtt ctttttttta agaattaatt aagcgaacga
451   gaataaagat agagtcccat tccacatgtc aataccggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgaccttt aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details