JRC GMO-Amplicons | EURL GMFF

Record ID:	KT124269.1
Database:	nt	Go to original record
Description:	Pinguicula vulgaris subsp. vulgaris isolate VU_NOR_2_a trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Pinguicula vulgaris subsp. vulgaris
Original size:	1580 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	680	1237	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    cctactaagt gataactttc aaattcagag aaaccccgga attaataaaa
101   atgggcaatc ctgagccaaa tcctgttttc tcaaaagaaa ggttccgaaa
151   acgaaaaaaa aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatggg gttgaccgcg atggtagagg aatctttccg ccgaaacttc
251   agagaggatc aaggataaac atatctattg aatactatat caaatgatta
301   ctgatggcac gaatctctat ttttatatga aaaatggaag aattggtgtg
351   aattgattcc acattgaaga aaaatcgaat atttattcat caaaccattc
401   actccatagt ccgatatacc ttttaacgaa ctgatcaatc ggacaagaat
451   aaagatagag tcccattcta catgtcaata ccggcaacaa tgcaatttat
501   agtaagagga aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct
551   ctatcccc

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details