JRC GMO-Amplicons | EURL GMFF

Record ID:	KT124240.1
Database:	nt	Go to original record
Description:	Pinguicula vulgaris subsp. bicolor isolate BI_SK_1_b trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Pinguicula vulgaris subsp. bicolor
Original size:	1580 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	679	1237	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    cctactaagt gataactttc aaattcagag aaaccccgga attaataaaa
101   atgggcaatc ctgagccaaa tcctgttttc tcaaaagaaa ggttccgaaa
151   acgaaaaaaa aaaaaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg ggttgaccgc gatggtagag gaatctttcc gccgaaactt
251   cagagaggat caaggataaa catatctatt gaatactata tcaaatgatt
301   actgatggca cgaatctcta tttttatatg aaaaatggaa gaattggtgt
351   gaattgattc cacattgaag aaaaatcgaa tatttattca tcaaaccatt
401   cactccatag tccgatatac cttttaacga actgatcaat cggacaagaa
451   taaagataga gtcccattct acatgtcaat accggcaaca atgcaattta
501   tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details