Match details
| Record ID: | LC094335.1 | |
|---|---|---|
| Database: | nt | Go to original record |
| Description: | Gateway recycling vector R4pRED56-MD8-53-R4R2R1R3 DNA, complete sequence. | |
| Taxon: | Gateway recycling vector R4pRED56-MD8-53-R4R2R1R3 | |
| Original size: | 8409 bp | |
View other 5 matches for this record
| Method: | QL-ELE-00-013 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim T-nos) (Waiblinger et al., 2008). | |
| Target: | T-nos | |
View other 15584 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 0 | 0 | 3643 | 3726 | + | 84 bp |
| Amplicon: | 84 bp |
|---|
1 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc 51 cgcaattata catttaatac gcgatagaaa acaa
View other 30799 matches with an identical amplicon
Primers and probe alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 25 bp | 0 | 0 | 1 | 25 | 9.20e-12 |
| primerB | 100.0 | 25 bp | 0 | 0 | 84 | 60 | 9.20e-12 |
| probe | 100.0 | 28 bp | 0 | 0 | 28 | 55 | 2.28e-13 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.