JRC GMO-Amplicons | EURL GMFF

Record ID:	KT750315.1
Database:	nt	Go to original record
Description:	Scaevola platyphylla tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Scaevola platyphylla
Original size:	951 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	549	+	549 bp

Amplicon:

549 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaacccagg aattaataaa
101   aaggggcaat cctgagccaa atcgcgtttt cagaaaacaa ttaaagtcag
151   aaagcgcaaa aaaaaggata ggtgcagaga ctcgatggaa gctgttctaa
201   cgaatggagt gaattgcctt gcgttggtag aggaatcctt atattgaaat
251   ttccgaaaag atcaagtata acctatatta tgcataatag aatataaata
301   atatatgcct aatagaattt caatttaaat atagaagaag tgttgtgaat
351   cgattcattc catatcggaa tattcatcga tcaaatcatt cactccataa
401   tctgatagat cttttgaaga agtgattaat cggacgagaa taaagataga
451   gtcctcttct acatgtctat accggcaata atgaaattta tagtaaaagg
501   aaaatccgtt gatttaaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	549	530	2.47e-08

Match details