JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ628315.1
Database:	nt	Go to original record
Description:	Dodartia orientalis chloroplast, complete genome.
Taxon:	Dodartia orientalis
Original size:	154271 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48077	48647	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt cactgcattg gtagaggaat ctttacaccg aaacttcaga
251   aaggatgaag gataaacgta tctattgaat actatatcaa ttaatgatgg
301   cccgaatctg tatctgtatt ttttaatctg aaaagtggaa gaattggtgt
351   gaattgattc cacattgaag aaagaatcga atatcgaata ttaattcatc
401   aaatcattga ctccatacat agtccgacag atcttttaaa gaactgatta
451   atcggacgag aataaagata gagtcccatt ctacatgtca atatcggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttat aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details