JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ628308.1
Database:	nt	Go to original record
Description:	Erythranthe tenella chromoplast, complete genome.
Taxon:	Erythranthe tenella
Original size:	154301 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48070	48637	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aagtaagaaa
101   aacgggcaat cctgagccaa atcctctttt ttcaaaagaa aggtttagaa
151   aacgaaaaaa aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttgattgcgt tggtagagga atctttccat cgaaacttca
251   gaaaggatga aggataaacg tatctattgt atatcaaatg attaatgata
301   gcctgatatc tgtatctgta ttttttaatt ttaagatgaa aaatgaaaga
351   attggtgtga attgattcca cattgaaaaa agaatgaaat attcattcat
401   caaatcattc actccatagt ccaatagatc ttttaaagaa ctgattaatc
451   ggacgagaat aaagatagag tcccgttcta catgtcaata ccggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details