JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ621980.1
Database:	nt	Go to original record
Description:	Bupleurum boissieuanum voucher zhc20111019-15-2 chloroplast, complete genome.
Taxon:	Bupleurum boissieuanum
Original size:	155803 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48793	49368	+	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg acttaataaa
101   aatgggcaat cctgagccaa atcctttttt taatttacaa aaacaaacaa
151   aggcccagaa ggtgaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaactg gaattgactg tgttgcattg gtagaggaat ccttccattg
251   aaacttccga aaagatgaag gatgtacgta tatacatacg tactgaaata
301   ctctatcaaa tgattaatga cgacctgcat ctgtattttt tatatgaaaa
351   acggaaaaat tgttgggaat cgattccata ttgaagaaag aatcgaatat
401   gcattgatca aagcatttac ctcacggtct gatagttctt ttgtataact
451   aattaatcgg acgagaataa agatagagtc ctattctaca tgtcattacc
501   ggcaacaatg aaatttatag taagaggaaa atccgttgat tttaaaaatc
551   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details