JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ442842.1
Database:	nt	Go to original record
Description:	Cucurbita moschata chloroplast, complete genome.
Taxon:	Cucurbita moschata
Original size:	157592 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	50204	50746	+	543 bp

Amplicon:

543 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aaggggtagg
151   tgcagagact caatggaagc tgttctaaca aatggagttg actacgttgc
201   gttagtaaag gaatgaatcc ttccaaactc cagaaaagat gaaagataaa
251   cgtattacgt actgaaatac tatattgatt aatgacaact cgaatctcta
301   ttttttatat ttatatataa aaaataatat ataaaaatga acgaattgtt
351   ataaatcgat gatcaaatca tttactccat catacagagt ctgatagatc
401   ttttgaagaa ctgattaatc agatgcgaat agaataaaga tagagtccca
451   ttctacatgt caataccgac aaaaatgaaa tttatagtaa gaggaaaatc
501   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	543	524	2.44e-08

Match details